Sample description
Organism:
mus
musculus [ NCBI taxonomy browser ]
Cell
source: in-house
bred mice (contact: norma.howells@itg.fzk.de)
Cell
type: thymocytes
(complex mixture of cells isolated from thymus. mostly T-lymphocytes and
epithelial cells of different differentiation stage) [no matching for this cell
type found in GXD "Mouse Anatomical Dictionary"]
Sex:
female
[ MGED ]
Age:
3 - 4
weeks after birth [ MGED ]
Growth
conditions: normal
controlled environment
20 - 22 oC
average temperature
housed in cages
according to German and EU legislation
specified pathogen free
conditions (SPF)
14 hours light cycle
10 hours dark cycle
Developmental
stage: stage
28 (juvenile (young) mice) [ GXD
"Mouse Anatomical Dictionary" ]
Organism
part: thymus
[ GXD "Mouse Anatomical Dictionary" ]
Strain
or line: C57BL/6
[International Committee on Standardized Genetic Nomenclature for Mice]
Genetic
Variation: Inbr (J) 150. Origin: substrains 6 and 10 were separated prior to 1937.
This substrain is now probably the most widely used of all inbred strains.
Substrain 6 and 10 differ at the H9, Igh2 and Lv loci. Maint. by J,N, Ola.
[International Committee on Standardized Genetic Nomenclature for Mice ]
Individual:
-
Individual
genetic characteristics: the same as in "Genetic Variation"
Disease
state: normal
Targeted
cell type: primary thymocytes (complex mixture of cells isolated from thymus.
mostly T-lymphocytes and epithelial cells of different differentiation stage [
no matching for this cell type found in GXD "Mouse Anatomical
Dictionary"]
Cell
line: no
cell line; primary cells
Treatment:
in vivo
[MGED] intraperitoneal
injection of Dexamethasone into mice, 10 microgram per 25 g bodyweight of the
mouse
Compound: drug [MGED] synthetic
glucocorticoid Dexamethasone, dissolved in PBS
Clinical
Information: -
Separation
technique: mice were killed 4 hours
post injection and the thymus was taken out. Thymozytes were prepared by
meshing the thymus through sterile gauze several times until a suspension of
individual cells was obtained.
RNA
was prepared from thymozytes using a Qiagen RNA preparation kit.